Punnett Square Worksheets

Genetics Blood Type
        Punnett square worksheets and genetics lesson plans that are designed for high school, middle school, and elementary school life science teachers are downloadable here for free. The Punnett square worksheets lets students practice the probability of inheritance. Mendel pea plant worksheets help students learn the history of genetics, the vocabulary of genetics and introduced to the probabilities of genetics. NGSS Life Science offers many great lessons. Free Lesson Plan (PDF) link below or become a member to get access to the answer key and editable file. Free Punnett square curriculum includes:
  • Worksheet Recently Updated
    Punnett Square Worksheet – Pea Plant Genetics
    Published by NGSS Life Science. Free Lesson Plan (PDF)

    Lesson Plan (DOCX) & Answer Key with Membership
     
    High School Worksheet
    Students will learn about the genotypes, phenotypes, and probabilities by completing this Punnett square worksheet for the different characteristics of pea plants.
    NGSS Standard
    HS-LS3-3
    		  
    Published by NGSS Life Science
  • Worksheet Recently Updated
    Punnett Square Worksheet - Fruit Fly Genetics
    Published by NGSS Life Science. Free Lesson Plan (PDF)

    Lesson Plan (DOCX) & Answer Key with Membership
     
    High School Worksheet
    In this Punnet square worksheet, students will learn about the genotypes, phenotypes, and probabilities of genetics for different characteristics of fruit flies.
    NGSS Standard
    HS-LS3-3
    		  
    Published by NGSS Life Science
  • Worksheet Recently Updated
    Dihybrid Cross Worksheet
    Published by NGSS Life Science. Free Lesson Plan (PDF)

    Lesson Plan (DOCX) & Answer Key with Membership
     
    High School Worksheet
    In this dihybrid cross worksheet, students will practice performing two trait crosses. Students will cross different parental genotypes and determine the offspring phenotype, and probabilities of having each trait. The last question asks students to make the connection between multiple traits and Mendel’s law of independent assortment.
    NGSS Standard
    HS-LS3-3
    		  
    Published by NGSS Life Science
  • Worksheet
    Genetics with SpongeBob - Dihybrid Crosses
    Free Lesson Plan
     
    High School Worksheet
    Students complete dihybird crosses about SpongeBob. SpongeBob SquarePants and all related characters are trademarks of Viacom International Inc.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Andrea Stonebraker
  • Lab Experiment
    Blood Type Genetics Lab
    Free Student Handout
    Free Teacher Notes
     
    High School Lab
    In this blood typing lab, students pretend to investigate blood samples (actually simple chemicals) and use their analysis of the experiment to solve a crime and determine if two babies were switched in the hospital.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Ingrid Waldron and Jennifer Doherty
  • Lab Experiment
    Independent Assortment and Gene Linkage Lab - Dragon Genetics
    Free Student Handout
    Free Teacher Notes
     
    High School Lab
    Students learn the principles of independent assortment and gene linkage in activities that analyze the inheritance of multiple genes on the same or different chromosomes in hypothetical dragons. The concepts of independent assortment of chromosomes during meiosis and random fertilization are modeled with linked genes.
    NGSS Standard
    HS-LS1-4
    HS-LS3-3
    		  
    Published by Ingrid Waldron and Jennifer Doherty
  • Worksheet Recently Updated
    Autosomal Pedigrees Worksheet
    Published by NGSS Life Science. Free Lesson Plan (PDF)

    Lesson Plan (DOCX) & Answer Key with Membership
     
    High School Worksheet
    Students will learn how to use pedigrees to trace a autosomal genes as they pass from generation to the next generation. Students will also learn about genetic disorders.
    NGSS Standard
    HS-LS3-3
    		  
    Published by NGSS Life Science
  • Worksheet
    Genetics with SpongeBob - Incomplete Dominance
    Free Lesson Plan
     
    High School Worksheet
    This worksheet provides several practice problems related to incomplete dominance with Poofkins (a special kind of flower) and Goobers (a special breed of jellyfish!) An answer key is provided. SpongeBob SquarePants and all related characters are trademarks of Viacom International Inc.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Tracy Trimpe
  • Test or Quiz
    SpongeBob Genetics Quiz
    Free Lesson Plan
     
    High School Test
    This SpongeBob genetics quiz includes heterozygous, homozygous, dominant, recessive, phenotype, genotype, and Punnett squares. SpongeBob SquarePants and all related characters are trademarks of Viacom International Inc.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Tracy Trimpe
  • Worksheet
    Punnett Square Genetics with SpongeBob #2
    Free Lesson Plan
     
    High School Worksheet
    A second helping of Bikini Bottom fun exploring the genetics of SpongeBob and his pals! An answer key is provided. SpongeBob SquarePants and all related characters are trademarks of Viacom International Inc.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Tracy Trimpe
  • Worksheet
    Punnett Square Genetics with SpongeBob #1
    Free Lesson Plan
     
    High School Worksheet
    What happens when SpongeBob SquarePants marries SpongeSusie RoundPants? Explore the genetics of Sponge Bob and his pals with this fun worksheet! An answer key is provided! SpongeBob SquarePants and all related characters are trademarks of Viacom International Inc.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Tracy Trimpe
  • Lab Experiment
    Genetics Lab - Introduction
    Free Student Handout
    Free Teacher Notes
     
    High School Lab
    In this genetics lab, students are introduced to genetics vocabulary, including meiosis, fertilization, Punnett squares, and pedigree analysis. Students flip coins to mimic the probability of genetics and use the coin flip results to answer inheritance questions.
    NGSS Standard
    HS-LS3-3
    		  
    Published by Ingrid Waldron and Jennifer Doherty
  • Worksheet Recently Updated
    Punnett Square Worksheet - Human Genetic Disorders
    Published by NGSS Life Science. Free Lesson Plan (PDF)

    Lesson Plan (DOCX) & Answer Key with Membership
     
    High School Worksheet
    In this Punnett square worksheet, students will learn about human genetic disorders for autosomal, sex-linked, pleiotropy, and incomplete dominant traits. Students will cross different parental genotypes and determine the offspring genotype, phenotype, and probabilities of having the genetic disorder and being a carrier of the genetic disorder.
    NGSS Standard
    HS-LS3-3
    		  
    Published by NGSS Life Science
  • Lab Experiment
    Mendelian Genetics Lab - Dragon Genetics
    Free Student Handout
    Free Teacher Notes
     
    High School Lab
    In this genetics lab, students use Popsicle sticks to represent a pair of homologous chromosomes with multiple genetic traits. Teams of students use their sets of Popsicle sticks to model fertilization and then identify the genetic makeup and phenotypic traits, resulting in a fictitious baby dragon.
    NGSS Standard
    HS-LS3-3
    MS-LS3-2 (Types of Reproduction)
    		  
    Published by Ingrid Waldron and Jennifer Doherty
Fruit Fly Genetics


Punnett Square Concepts

  • Classical Genetics - Gregor Mendel, inheritance, heredity of traits, pea plants experiments.
  • Punnett Squares - genotype, phenotype, genotypic ratios, phenotypic ratios
  • Monohybrid Cross Punnett Squares - pea plant genetics, genetic disorders, genetic probability with a single trait.
  • Dihybird Cross Punnett Squares - fruit fly genetics, genetic probability with two traits.
  • Advanced Punnett Squares - incomplete dominance, pleiotropy, autosomal traits, sex-linked traits.
  • Mendel’s Law of Dominance - dominant, recesive, carrier of a trait, dominant allele, recessive allele.
  • Law of Segregation - Gene, allele, homozygous, heterozygous, gametes.
  • Law of Independent Assortment - multi-trait crosses, 9:3:3:1 ratio, chromosomes assort independently.
  • Genetic Disorders - cystic fibrosis, albinism (albino), neurofibromatosis, sickle-cell anemia, hemophilia A, color blindness.
  • Gametes - sperm, egg, ovum, gonads.
  • Pedigrees - autosomal and sex-linked.


Lessons Organized by NGSS Standard